anti-epcam antibody-coated magnetic particles using the ctc profiler  (CellSearch inc)

Journal: Translational Cancer Research

Article Title: IL-6 promotes metastasis and EMT of non-small cell lung cancer cells by up-regulating FGL1 via STAT3 pathway

doi: 10.21037/tcr-2025-119

Figure Lengend Snippet: NSCLC patients with high FGL1 expression have higher clinical stage and poor prognosis. The association of FGL1 expression with the clinical stage (A), survival analysis (B) and OS (C) of NSCLC patients was analyzed using a public database ( https://www.cancer.gov/ccg/research/genome-sequencing/tcga ). (D) According to the standard procedure of CTC extraction, tumor cells circulating in the venous blood of NSCLC patients were isolated, and FGL1 + CTCs were detected by immunofluorescence (Bar, 10 µm). Dynamic changes of FGL1 + CTC count (E) and chest CT imaging (F) in NSCLC patients were analyzed before and after treatment. Arrows indicate target lesions that can be used for observation and comparison. CT, computed tomography; CTC, circulating tumor cells; DFS, disease-free survival; HR, hazard ratio; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; NSCLC, non-small cell lung cancer; OS, overall survival; TPM, transcripts per million.

Article Snippet: CD45 + cells were specifically removed using a CD45 kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), and the remaining cells were divided into two portions and separated by magnetic beads into CSV + CTCs (H00007431-MF08; Abnova, Taiwan, China) and EPCAM + CTCs (2929; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing, Sequencing, Extraction, Isolation, Immunofluorescence, Imaging, Comparison, Computed Tomography

Journal: Frontiers in Molecular Biosciences

Article Title: Prospects of liquid biopsy in the prognosis and clinical management of gastrointestinal cancers

doi: 10.3389/fmolb.2024.1385238

Figure Lengend Snippet: Representative studies relating circulating tumor cell (CTC) detection for various clinical applications in gastrointestinal (GI) tract cancers.

Article Snippet: , Metastatic GC , Anti-EpCAM antibody-coated magnetic particles using the CTC Profiler (Veridex) , >5 CTCs per 5 mL of blood , During the start of various chemotherapy cycles , Positive CTC score associated with unfavorable PFS and OS.

Techniques: Immunostaining, Diagnostic Assay

Journal: Frontiers in Molecular Biosciences

Article Title: Prospects of liquid biopsy in the prognosis and clinical management of gastrointestinal cancers

doi: 10.3389/fmolb.2024.1385238

Figure Lengend Snippet: Studies relating CTC detection and various prognostic parameters in gastrointestinal cancers.

Article Snippet: , Metastatic GC , Anti-EpCAM antibody-coated magnetic particles using the CTC Profiler (Veridex) , >5 CTCs per 5 mL of blood , During the start of various chemotherapy cycles , Positive CTC score associated with unfavorable PFS and OS.

Techniques: Imaging, Immunostaining, Isolation, Immunofluorescence, Immunomagnetic Separation

Journal: Journal of Pharmaceutical Analysis

Article Title: Recent progress in aptamer-based microfluidics for the detection of circulating tumor cells and extracellular vesicles

doi: 10.1016/j.jpha.2023.03.001

Figure Lengend Snippet: Multiple-target aptamer-based microfluidics for the detection of circulating tumor cells (CTCs). (A) Combining multiplex surface-enhanced Raman scattering (SERS) nanovectors and multivariate analysis for in situ profiling of CTC phenotypes using a microfluidic chip. (B) Simultaneous single-cell phenotype analysis of hepatocellular carcinoma CTCs using a SERS-aptamer-based microfluidic chip. (C) A poly(lactic- co -glycolic acid) (PLGA) nanofiber microfluidic device for highly efficient isolation and release of CTCs with different phenotypes based on dual aptamers. (D) Dual-multivalent-aptamer-conjugated nanoprobes for superefficient discerning of single CTCs in a microfluidic chip with inductively coupled plasma-mass spectrometry (ICP-MS) detection. EGFR: epidermal growth factor receptor; EpCAM: epithelial cell adhesion molecule; HER2: human epidermal growth factor receptor-2; HCC: hepatocellular carcinoma; WBC: white blood cells; RBC: red blood cells; MGITC: malachite green isothiocyanate; DTDC: 3,3′-diethylthiadicarbocyanine iodide; AuNPs: gold nanoparticles; PDMS: polydimethylsiloxane; PLGA: poly(lactic- co -glycolic acid); F i : inertial lift forces; F d : dean drag force; DMA: dual-multivalent-aptamers. Reprinted from Refs. [ , , , ] with permission.

Article Snippet: SYL3C , EpCAM , MCF-7 , CTCs and single-target simple aptamer-based microfluidics , Aptamer-modified magnetic nanoclusters; biomimetic microfluidic device; and fluorescence analysis , [ ] .

Techniques: Multiplex Assay, In Situ, Isolation, Clinical Proteomics, Mass Spectrometry

Journal: Journal of Pharmaceutical Analysis

Article Title: Recent progress in aptamer-based microfluidics for the detection of circulating tumor cells and extracellular vesicles

doi: 10.1016/j.jpha.2023.03.001

Figure Lengend Snippet: Aptamer-based microfluidic for the detection of circulating tumor cells (CTCs) and extracellular vesicles (EVs).

Article Snippet: SYL3C , EpCAM , MCF-7 , CTCs and single-target simple aptamer-based microfluidics , Aptamer-modified magnetic nanoclusters; biomimetic microfluidic device; and fluorescence analysis , [ ] .

Techniques: Fluorescence, Modification, Isolation, In Situ

Journal: Journal of Pharmaceutical Analysis

Article Title: Recent progress in aptamer-based microfluidics for the detection of circulating tumor cells and extracellular vesicles

doi: 10.1016/j.jpha.2023.03.001

Figure Lengend Snippet: Multiple-target aptamer-based microfluidics for fluorescent detection of extracellular vesicles (EVs). (A) A microfluidic coflow system using two labelled aptamers with Cy5-conjugated human epidermal growth factor receptor-2 (HER2) aptamer and fluorescein amidite (FAM)-conjugated epithelial cell adhesion molecule (EpCAM) aptamer to visualize the separation process. (B) A thermophoretic aptasensor using a panel of seven fluorescent Cy5-conjugated aptamers to label serum EVs. MVB: multivesicular body; EXO: exosomes; MVs: microvesicles; ABs: apoptotic bodies; TBE: tris-boric acid-ethylenediamine tetraacetic acid; F e : the centerline-directed elastic lift force; F i : inertial lift force; F d : viscous drag force; a: diameter of EV; LDA: linear discriminant analysis; sCMOS: scientific complementary metal-oxide semiconductor. Reprinted from Refs. [ , ] with permission.

Article Snippet: SYL3C , EpCAM , MCF-7 , CTCs and single-target simple aptamer-based microfluidics , Aptamer-modified magnetic nanoclusters; biomimetic microfluidic device; and fluorescence analysis , [ ] .

Techniques: